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PCR Featured Raw Materials

Date: 2019-01-22

DNA Related


Taq

(MD001)

Platinum

(MD002)

Anstart Taq

(MD006)

Taq II DNA Polymerase

(MD0032)

AnPfu

Hot start

(MD036)

Hot start

HItaq

(MD026)

Anstart Taq DNA II Polymerase

(MD007)

Performance

Speed (Max in Internal test)

4KB/1min;

(1KB/5s*)

4KB/1min;

(1KB/5s*)

4KB/1min;

(1KB/5s*)


1KB/30s

1KB/min

1KB/min

Heat activation

95

2.5min

95

2.5min

95

2.5min


95

2.5min

95

10min

95

10min

Hot start





Amplicon size

gDNA


3.2KB





3.2KB

Plasmid/lambda

3KB

3KB

3KB



3KB

3KB

cDNA

5KB

5KB

5KB


8KB

5KB

5KB

Application

Low Copy Number


Routine PCR


T/A cloning


Colony PCR

Sequencing



Genotyping




Multiplex PCR



√*

√*


√*

RT-PCR




Complex Template



√*

√*

GC-Rich






Blunt -end cloning







Mutagenesis







Additional Features

5'–3' polymerase

activity


3'–5'exo-nuclease activity







Isothermal Related


Bst DNA PolymeraseMD030

Phi29 DNA PolymeraseMD031

Performance

Optimal extension temperature

60-65

30

Heat activation

80,20min

65,10min


Specification

Isothermal DNA amplification

Isothermal amplification with Hi-Fi

Sequencing

Trace DNA / GC rich sequencing

Strand replacement/leading strand synthesis/PGSPGD*

* : Optimized with tailor-made buffer 


RNA Related


2.5 X SYBR Anstart One-Step RT-PCR Mix (MD013G)

Anstart One-Step RT-PCR Mix (MD013)

Hot start HITaq One-Step RT-PCR Mix (MD027)

M-MuLV Reverse Transcriptase

MD011

Super

M-MuLV Reverse TranscriptaseMD028

Tth DNA PolymeraseMD012


Polymease

Type

Anstart Taq

Hot start Taq

Hot start HITaq





Detection method

SYBR Green 1

probe

probe





Hot start type

antibody

chemical

chemical





Amplicon size




12KB

12KB

2-3KB


Heat activation







95

2.5min

Optimal Extension temperature




50

50

60-65


Format

1 step

(RNA -Ct)

1 step

(RNA -Ct)

1 step

(RNA -Ct)






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