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FAPON PCR raw materials

Date: 2017-10-26

DNA related


Taq

(MD001)

Platinum

(MD002)

Anstart Taq

(MD006)

Anstart mix (MD008A)

Anstart Master mix(MD009A**)

2x SYBR Anstart Master Mix (MD008G)

AnPfu

Hotstart

(MD036)

Hotstart

HItaq

(MD026)

Hotstart HITaq mix

(MD008H)

Performance

Speed (Max in Internal test)

4KB/1min;

(1KB/5s*)

4KB/1min;

(1KB/5s*)

4KB/1min;

(1KB/5s*)


1KB/30s

1KB/min


Heat activation

95

2.5min

95

2.5min

95

2.5min


95

2.5min

95

10min


Hotstart



Amplicon size

gDNA


3.2KB






Plasmid/lambda

3KB

3KB

3KB



3KB


cDNA

5KB

5KB

5KB


8KB

5KB


Application

Low Copy Number


Routine PCR


T/A cloning


Colony PCR


Sequencing


Genotyping




Multiplex PCR



 √*

 √*


 √*

RT-PCR




Complex Template



 √*

 √*


GC-Rich 






Blunt -end cloning







Mutagenesis







Additional Features

5'–3' polymerase

activity



3'–5'exo-nuclease activity







   **: Good pollution prevention with UDG enzyme

    * : Optimized with tailor-made buffer


Isothermal related


Bst DNA PolymeraseMD030

Phi29 DNA PolymeraseMD031

Performance

Optimal extension temperature

60-65

30

Heat activation

80,20min

65,10min


Specification

Isothermal DNA amplification

Isothermal amplification with Hi-Fi

Sequencing

Trace DNA / GC rich sequencing

Strand replacement/leading strand synthesis/PGSPGD*

 

RNA related


2.5 X SYBR Anstart One-Step RT-PCR Mix (MD013G)

Anstart One-Step RT-PCR Mix (MD013)

Hotstart HITaq One-Step RT-PCR Mix (MD027)

M-MuLV Reverse Transcriptase

(MD011)

Super

 M-MuLV Reverse TranscriptaseMD028

Tth DNA PolymeraseMD012

RNasinMD015

Polymease

Type

Anstart Taq

Hotstart Taq

Hotstart HITaq





Detection method

SYBR Green 1

probe

probe





Hotstart type

antibody

chemical

chemical





Amplicon size




12KB

12KB

2-3KB


Heat activation






95

2.5min


Optimal Extension temperature




50

50

60-65


Format

1 step

(RNA -Ct)

1 step

(RNA -Ct)

1 step

(RNA -Ct)





* : Optimized with tailor-made buffer


Sequencing library preparation

Library preparation kit and module

MD068H

End-Repair Mix (High Concentration)

MD068L

End-Repair Mix (Low Concentration)

MD069

NGS Library Prep: 2XHiFi Seq PCR Mix

MD070

Fapon DNA Sample Prep Kit for Illumina Platform

MD072

Fapon Library Quantification Kit for Illumina Platform

MD073

Multi Enzyme Mix

FPZ-02

Fapon DNA Sample Prep Kit for Ion Torrent Platform

FPZ-03

10x Uracil Clear System

FPZ-04

5x WGS Fragmentation Mix

Raw material for NGS and single-cell seq library preparation

MD001

Taq DNA Polymerase

MD014

Uracil DNA Glycosylase

MD015

RNAse Inhibitor

MD029

Heat-labile Uracil-DNA Glycosylase

MD030

Bst DNA Polymerase

MD031H

Phi29 DNA Polymerase(High Concentration)

MD031L

Phi29 DNA Polymerase(Low Concentration)

MD033

T4 DNA polymerase

MD034

T4 Polynucleotide Kinase

MD035

Fast T4 DNA Ligase

MD036

AnPfu Hotstart DNA Polymerase

MD037

Klenow Fragment

MD038H

Klenow (3′→ 5′ exo-) (High Concentration)

MD038L

Klenow (3′→ 5′ exo-) (Low Concentration)

MD050

Phi29 DNA Polymerase(3′→ 5′ exo-)

MD056

RNAse H

MD063

HiFi Seq DNA Polymerase

MD065

WGS Ligase

FPZ-09

T7 DNA Polymerase

FPZ-14

T7 RNA Polymerase

FPZ-16

T3 DNA Ligase

FPZ-20

Taq DNA Ligase

FPZ-17

T4 DNA Ligase

FPZ-21

T4 RNA Ligase1

FPZ-22

T4 RNA Ligase2

FPZ-23

T4 RNA Ligase2 Truncated

FPZ-24

T4 Gene 32 Protein

FPZ-26

DNA Polymerase I

FPZ-29

Poly(A) Polymerase

FPZ-31

Exonuclease I

FPZ-32

Exonuclease III

FPZ-33

Endonuclease VIII

FPZ-34

Lambda Exonuclease

FPZ-35

RecA

FPZ-38

E. coli DNA Ligase

FPZ-39

E. coli fpg

FPZ-40

E. coli Pyrophosphatase

FPZ-41

E. coli Single-Stranded DNA Binding Protein

FPZ-42

Terminal deoxynucleotidyl Transferase (TdT)

FPZ-44

Thermostable Pyrophosphatase

FPZ-47

HiFi Seq ultra DNA Polymerase

 

OTHERS

No.

Product name

Description

dN(U)TP mix

MD016

25mM dNTP mix(100mM dA:dC:dG:dT=1:1:1:1)

High purity

MD016M

25mM dN(U)TP mix(100mM dA:dC:dG:dU=1:1:1:1)

MD016L

20-40mM dN(U)TP mix(100mM dA:dC:dG:dU=1:1:1:2)

dN(U)TP

MD017

100mM dATP

High purity

MD018

100mM dCTP

MD019

100mM dGTP

MD020

100mM dTTP

MD021

100mM dUTP

Functional Enzymes

MD014

Uracil-DNA Glycosylase(UDG)

To prevent nonspecific PCR amplification

MD025

Proteinase K

High purity

MD022

SA-HRP/SA-POD

Low background and high sensitivity


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