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Whole Blood PCR Kit

Code

Product Name

MD039

Whole Blood PCR Kit


MD039-- Whole Blood PCR Kit

WB DNA Polymerase is a high-performance, blood direct amplification PCR product, with the function resists the inhibitory effects of higher concentrations of blood (EDTA, heparin, sodium citrate), SDS, and NaCl. Even in the absence of a PCR enhancer and in certain concentrations of blood samples where other commercial enzymes fail, they still work in 40% whole blood (50ul system) PCR.


Amplification will be better in the presence of PCR enhancers. It can be directly amplified from whole blood prior to PCR without DNA purification, can be hot-started, and yields high. This new hot-start enzyme preparation from Fapon is equipped with a 5xWB buffer (with Mg ), which is a proprietary reaction buffer containing 2xenhancer components. It’s not suggested to add enhancer in the first experiment. If the amplification effect is poor, then add the enhancer to debug. It should be noted that WB DNA Polymerase contains a certain amount of calibration activity, and the amplified product is blunt. It is necessary to purify the amplification product and finish A tailing before TA cloning.