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Reverse Transcription PCR

Code

Product Name

MD011

M-MuLV Reverse Transcriptase

MD028

Super M-MuLV Reverse Transcriptase

MD012

Tth DNA Polymerase

MD015

RNASE Inhibitor


MD011-- M-MuLV Reverse Transcriptase

M-MuLV Reverse Transcriptase is an RNA-dependent DNA polymerase that mainly used for long mRNAs to cDNA synthesis. Because of a deficient RH activity, it replaces the AMV reverse transcriptase and becomes one of the preferred products during long fragments of cDNA synthesis. By genome editing, Fapon reduced the RH activity to the minimum with robust reverse transcription performance.


The enzyme is in high purity, no ribonuclease activity, no nuclease contamination and no other plasmid contamination. the efficiency of full-length molecules synthesis is greater than 95%, with a favorable price.


Advantages of M-MuLV Reverse Transcriptase 

  1. Lacking RNase H activity: depletion of reduced RNase H activity is beneficial to obtain more full-length cDNA;

  2. Good thermal stability: the optimum reaction temperature is 50°C, and the reverse transcription can be performed up to 60°C, it effectively overcomes the secondary structure of template RNA and completes the reverse transcription experiment successfully.

  3. Wide application temperature: reverse transcription can be performed in the range of 37°C-60°C, it still keep over 80% of the highest activity even in the range of 42°C-55°C. Users can select the reaction temperature according to their needs;

  4. Strong amplification activity: mutations improve the binding ability of reverse transcriptase and RNA, increase the amplification rate of the enzyme, and can obtain higher-quality cDNA, meeting the requirements for the construction of a cDNA library.


MD028---Super M-MuLV Reverse Transcriptase

Super M-MuLV improved its specificity in order for the highest yield of cDNA in all reverse transcriptase, it is also suitable for cDNA which reverses transcripted from longer mRNA. Due to low RH activity, super M-MuLV possesses better catalytic action than other AMV reverse transcriptase in long cDNA catalyzation. Furthermore, its advantage is outstanding for some special gene's RT-PCR or cDNA from total RNA or poly(A) and RNA.

Fapon introduces specific site directed mutagenesis into the super M-MuLV, which would not only extend the half-life period, but also lower RNase activity and enhance stability. Good reverse transcription ability was observed in a series of strict demonstration tests and QC experiments.


MD012---Tth DNA Polymerase

Tth DNA polymerase is a thermostable DNA polymerase. The enzyme has a reverse transcriptase activity in addition to a 5'→3' polymerase activity and a double strand specific 5'→3' exonuclease activity in the presence of Mn ions. Therefore, this enzyme enables ‘one-step RT-PCR’ including the reverse transcription and PCR steps. Kits for one-step RT-PCR and real-time PCR using this enzyme are also available.


MD015--- RNase Inhibitor

RNasin can specially compound with RNase based on covalent bond, which makes RNase non-active. RNasin can protect mRNA's integrity and improve efficiency of transcription and translation. At the same time, it avoids the affection which comes from using organic compounds' inhibitor. This product is electrophoresis pure, without RNase and Nickase pollution. RNasin specific activity meets the international criterion.

Rnasin inhibits eukaryotic organism’s activity: RNase A/B/C, and Placenta.

Rnasin does not inhibit Rnase H/S1 Nucleic acid enzyme, SP6, T7 and T3RNA Polymerase, AMV or M-MLV Reverse transcriptase, Taq DNA Polymerase, RNase T1. It doesn't Transcription and Protein translation. Buffer system needs to have 1mMDTT exist, the wide range of active Ph.

Application:

  1. Prevent potential RNase contamination

  2. Protecting mRNA in CDNA compounded/Vitro Transcription Systems/Translation Systems

  3. Improve Polysomes' activity and output; Virus vitro copy

  4. Produce Non-RNase protein product, such as antibody