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Library Preparation Module

DNA Library Construction

Code

Product Name

MD070-E

End-Repair Module

MD070-A

A-Tailing Module

MD073-E

End-Repair & A-Tailing Module

MD070-L

Adapter Ligation Module

MD065

WGS Ligase

FPZ-04

WGS Fragmentation Mix


MD070-E -- End-Repair Module

Fapon DNA End-repair module is applied to repair cohesive ends of fragmental dsDNA and form 5’ phosphorylated and 3’-OH blunt ends. dsDNA amount from 10ng to 5ug can be handled with this End-repair module.


Application Scope: Fragmental gDNA、cDNA、cfDNA、ctDNA and other PCR products after enzymatic or physical shearing.



Item

End-Repair Module

End Repair buffer 

End Repair Enzyme Mix


MD070-A-- A-Tailing Module

Fapon A-tailing module is applied to the adenine adding of 3’ end of fragmental DNA during DNA library construction. dsDNA amount from 10ng to 5ug is manageable with this end-repair module.


Application Scope: DNA processed by end-repair module and blunt-end dsDNA



Items


A-Tailing Module

 

5×A-Addition Buffer


A-Tailing Enzyme




MD073-E -- End-Repair & A-Tailing Module 

This module is applied to repair cohesive ends of fragmental dsDNA and form 5’ phosphorylated and 3’-OH blunt-end. Furthermore, adding an adenine to 3’ end of fragmental DNA, dsDNA amount from 10ng to 5ug is manageable with this End-repair module.


Application Scope: Fragmental gDNA、cDNA、cfDNA、ctDNA and other PCR products after enzymatic or physical shearing.



Items

End-Repair module

End Repair & A-tailing buffer 

End Repair & A-tailing Enzyme Mix


MD070-L—Adapter Ligation Module

Fapon DNA fast ligation module is applied to connect complementary cohesive end or blunt-end dsDNA with adapters. dsDNA amount from 10ng to 5ug can be handled with this End-repair module.


Application Scope: 

1) dsDNA processed with A-tailing module / T vector

2) dsDNA processed with end-repair & A-tailing module / T vector 

3) complementary cohesive end dsDNA 

4) Blunt-end dsDNA



Items


Fast ligation module

Ligation buffer


Ligation enzyme



NM002---WGS Fragmentation Mix

This mix provides a single-tube solution for library construction for Illumina platforms. The protocol supports fragmentation, end-repair and dA-tailing in a single reaction step, therefore greatly simplifying the workflow, reducing the total reaction time and hands-on time.