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Library Preparation Kit

DNA Library Construction

Code

Product

NK001

Tnp Library Prep Kit for Illumina

MD073

Fast DNA Library Prep Kit for Illumina(Two Steps)

MD070

DNA Library Prep Kit for Illumina(Three Steps)

MD079

RNA-seq Strand-specific Library Prep Kit

MD075

Single-Cell RNA sequencing library Prep Kit

NK004

DNA Library Prep Kit for Ion Torrent Platform


NK001--Tnp Library Prep Kit for Illumina

Fapon Tnp Library Prep Kit for illumina is prepared with Fapon’s new self-developed transposase, specially designed for the fast DNA library construction in Illumina platform. The kit offers fast and easy workflow, enabling 50ng different DNA sample types to be constructed within 100 mins. It is highly optimized with the quality control process to ensure the efficiency and stability of the library construction, making no GC content bias in the sample.


MD073-- Fast DNA Library Prep Kit for Illumina (Two Steps)

The Fapon DNA Sample Prep Kit is utilized for the construction of genomic DNA library. All optimized enzymes and buffers are provided with efficient and stable reagents, which can convert 10 ng to 5 ug of fragmented dsDNA into DNA libraries.

 

Application Scope:

Suitable for the preparation of DNA libraries for Illumina sequencing.  Applicable for a variety of sample types and species sources, including a variety of microbial genome, animal and plant genomes, human and mouse genomes.

 

Compatible with the following types of samples:

l  Genomic DNA

l  REPLI-g amplified DNA

l  Reverse transcription of double-stranded cDNA.

l  PCR Amplicons

l  Cell-Free DNAcfDNActDNA

l  DNA extracted from FFPE samples

l  ChIP Seq



Items

20 Reactions

End Repair & A tailing

End Repair & A-tailing buffer

250 μl

End Repair & A-tailing Enzyme Mix

120 μl

Adapter ligation

Ligation Buffer

750 μl

Ligation Enzyme

650 μl


Performance:

  • Shorten preparation time of DNA library to 3 hours. 

  • Less input of DNA templates is required

  • Product quality compatible with Kapa DNA library prep kit after a comparative test on conventional genome library construction.


MD070--DNA Library Prep Kit for Illumina (Three Steps)

The Fapon DNA Sample Prep Kit is utilized for the construction of genomic DNA library. All optimized enzymes and buffers are provided with efficient and stable reagents, which can convert 10 ng to 5 ug of fragmented dsDNA into DNA libraries.

 

Application Scope: Suitable for the preparation of DNA libraries for Illumina sequencing. Applicable for a variety of sample types and species sources, including a variety of microbial genome, animal and plant genomes, human and mouse genomes.

 

Compatible with the following types of samples:

l  Genomic DNA

l  REPLI-g amplified DNA

l  Reverse transcription of double-stranded cDNA.

l  PCR Amplicons

l  Cell Free DNAcfDNActDNA

l  DNA extracted from FFPE samples

l  ChIP Seq



Items

20 Reactions

End Repair

End Repair Buffer 

250 μl

End Repair Enzyme Mix

100 μl

A Tailing

5×A-Tailing Buffer 

250 μl

A-Tailing Enzyme Mix

120 μl

Adapter ligation

2×Ligation Buffer

600 μl

Ligation Enzyme

70 μl

Fapon three-step DNA library prep kit adopted the classical protocol of library construction. Purification at the end of each step to improve DNA purity, ensuring the efficiency of Library conversion to the maximum degree. Quality of Fapon’s kit equals to Kapa DNA library prep kit after a comparative test on conventional genome library construction.


MD079 -- RNA-seq Strand-specific Library Prep Kit

Fapon RNA Library Prep Kit is applied to transcriptome library preparation on Illumina high-throughput sequencing platform. All optimized enzyme and buffer are stored in a form of reagent. Only 0.1-4ug total RNA is required to construct RNA library.


Application Scope: 0.1-4ug human/animal/plant/microorganisim total RNA with good integrity. rRNA which is often not of interest in total RNA, can be removed using oligo dT beads, or can be depleted using rRNA specific probes.




Items

Fragmentation&

random primers

Fragmentation Buffer

Random Primers

First strand synthesis

First Strand Synthesis Reaction Buffer

First Strand Synthesis Enzyme Mix

Second strand synthesis

Second Strand Synthesis Reaction Buffer with dUTP Mix

Second Strand Synthesis Enzyme Mix

End repair and A-tailing

End Repair & A-Tailing buffer 

End Repair & A-Tailing Enzyme Mix

Adaptor ligation

Ligation Buffer

Ligation Enzyme


MD075--Single-Cell RNA Sequencing Library Prep Kit

Single-Cell RNA sequencing library Prep Kit can extract 10pg-10ng high-quality whole cDNA from single to 10 cells. The amplified products can be applied to Life and Illumina platform for library preparation.



Items

20 Reactions

Cell Lysis

Cell Lysis buffer

15 μl

RT-Primer

15 μl

First Strand Synthesis

RT Buffer

50 μl

RTase

20 μl

 

 

Second Strand Synthesis & PCR Amplification

Cut Buffer

μl

Cutter

15 μl

Tag Buffer

15 μl

Tagger

7.5 μl

PCR Buffer

1.25 ml

Tag-Primer

10 μl

DNA Polymerase

50 μl

PCR-Primer

25 μl


NK004---DNA Library Prep Kit for Ion Torrent Platform

Preparation of library DNA is an important process for successfμl amplification on Ion Torrent™ platforms and subsequently generates valuable sequencing data. This kit provides room temperature stable reagents to convert double-stranded fragmented DNA into libraries. The DNA Sample Prep Kit is primarily designed for constructing genomic DNA libraries. All enzymes and buffers required are provided as room temperature stable mixes that can convert 10ng to 1μg of fragmented dsDNA into library DNA.